For OFAC, a person subject to US jurisdiction “may be held civilly liable even if such person did not know or have reason to know that it was engaging in a transaction that was prohibited”, as the 2024 Tri-Seal Compliance Note puts it. Knowledge still matters, but as a factor in the penalty, not as a condition of the violation.
In the UK the Economic Crime (Transparency and Enforcement) Act 2022 amended the Policing and Crime Act 2017 on 15 March 2022, and for breaches on or after 15 June 2022 OFSI no longer has to show the person knew or had reasonable cause to suspect a breach. The knowledge then counts only as a case factor. The Apple Distribution International case turns on it: a payment processed on the day a designation was published was a breach, and OFSI treated the timing as mitigation rather than as a defence.